Foreign Ministry Spokesman Wang Wenbin said on Monday that the "bullying behavior" of the Trump administration was evident.
He slammed Washington for "abusing national power to unreasonably suppress other countries' enterprises."
China says Trump is strong-arming the company into giving up full ownership of a lucrative app -- with 100 million US subscribers -- to an American rival.
Wang said that instead of exerting pressure on the Chinese-owned company to sell its US operations, the US should "provide a fair, just, open, and non-discriminatory business environment for companies around the world investing and operating in the country."
President Donald Trump, however, claims the popular app poses a security threat to the US.
In the meantime, a US federal court issued a temporary block on a ban ordered by the US Commerce Department against Tilk Tok.
District Judge Carl Nichols in Washington late Sunday blocked the ban set to take effect at 11:59 p.m. Sunday.